Fig 1.
Effects of an HIF-1α activation/inhibition on the intestinal mucosa in septic rats, as shown by HE staining (×200) and the Chiu score of (n = 6 except sepsis + BAY 87–2243 with n = 5).
Data are expressed as the mean (SD). A. Representative images showing the intestinal morphology (HE staining, magnification × 200). B. Intestinal mucosa injury was quantitatively assessed by calculating the Chiu score; *P < 0.05 compared with the sham group; #P < 0.05 compared with the sepsis group.
Fig 2.
Effects of an HIF-1α activation/inhibition on inflammation and oxidative stress in septic rats (n = 6 except sepsis + BAY 87–2243 with n = 5).
Data are expressed as the mean (SD). Changes in IL-6 (A), IL-1β (B), and TNF-α (C) plasma concentrations; the MDA plasma content (D); and SOD (E) and CAT (F) activities in each group are depicted. *P< 0.05 compared with the sham group; #P < 0.05 compared with the sepsis group.
Fig 3.
Effects of an HIF-1α activation/inhibition on the levels of DAO (A), FABP2 (B), and D-lactac acid (C) and the fluorescent signal corresponding to FD4 (D) in the plasma of rats (n = 6 except sepsis + BAY 87–2243 with n = 5). Data are expressed as the mean (SD); *P < 0.05 compared with the sham group; #P < 0.05 compared with the sepsis group.
Fig 4.
Effects of an HIF-1α activation/inhibition on the protein expression of HIF-1α (B) and TJ proteins (C-E) in the intestinal mucosa of rats (n = 6 except sepsis + BAY 87–2243 with n = 5). Data are expressed as the mean (SD). A. Bands showing protein expression (three animals). B-E. Protein expression of HIF-1α, ZO-1, Occludin, and Claudin-1. *P < 0.05 compared with the sham group; #P < 0.05 compared with the sepsis group.
Table 1.
Effects of an HIF-1α activation/inhibition on the expression of HIF-1α and TJ proteins in the intestinal mucosa in rats.