Fig 1.
Increased body weight gain and development of glucose intolerance in high fat diet fed C57BL/6 mice.
C57BL/6 mice were fed a HFD, a matched purified ingredient control diet (CD), or a regular chow diet (ChD) for 10 weeks. (a) Average food consumption; #p<0.01 (n = 5); (b) Calorie intake throughout the experimental period and average intake *p<0.05, #p<0.01 (n = 5); (c) Body weight gain. Starting from week 3, body weights between HFD-fed and CD were significantly different at p<0.01; HFD versus ChD groups were also significantly different, although weeks 3, 4 and 5 at a lower p value (p<0.05); (d) Glucose tolerance test. Mice were fasted overnight and given a bolus of glucose (1 mg/g body weight). Blood glucose was monitored at the indicated time points. The indicated time points in the HFD-fed group were significantly different relative to the CD as well as ChD groups; (e) Fed blood glucose after 10 weeks of feeding the special diets; #p<0.01 (n = 5).
Table 1.
Non-fasting serum chemistries.
Fig 2.
Hepatic steatosis in C57BL/6 mice fed a HFD.
Livers from mice fed the CD or ChD diet showed no lipid accumulation. Mice fed the HFD had marked extensive macrovesicular steatosis. Hematoxylin & eosin, 100x; Oil Red O, 100x.
Fig 3.
cDNA-uPA/SCID mice do not develop insulin resistance upon HFD feeding.
cDNA-uPA/SCID mice were fed a HFD or CD for 12 weeks. (a) Body weight (n = 4); (b) GTT on week 12. Mice were fasted for 7 hours and a bolus of glucose (1 mg/g body weight) was given intraperitoneally. Blood glucose was measured at the indicated times (n = 4); (c) Fed blood glucose was measured 2 days after the GTT (n = 4).
Fig 4.
Liver and kidney histology in cDNA-uPA/SCID mice.
(a) cDNA-uPA/SCID mice fed a HFD or a CD develop hepatic steatosis regardless of diet. Similar amounts of lipid droplets were observed in the ORO staining. (b) Kidney abnormalities in cDNA-uPA/SCID mice. Glomeruli in kidneys of cDNA-uPA/SCID mice showed an increase in the mesangial matrix with narrowing of the capillary loops, and variable increase in size, as compared to C57BL/6 mice. Hematoxylin eosin, 100x; Oil Red O, 100x. (c) Lymphoma in cDNA-uPA/SCID mice. The normal splenic architecture is effaced by an infiltrate of medium sized, hyperchromatic, monotonous cells with a high nuclear-cytoplasmic ratio and scant cytoplasm. The nuclei displayed irregular and coarse membranes, and contained clumped chromation with inconspicious nucleoli. Numerous mitotic figures (arrows) are present (top). The liver (middle) is massively involved and the kidney (bottom) shows a similar infiltrate of monotonous, dyscohesive, hyperchromatic cells.
Table 2.
Liver lipids in cDNA-uPA/SCID mice.