Fig 1.
Changes in body weight, adipose tissue, and blood indexes in the hypoxia group versus the normoxia group.
Hemoglobin, (B) 0-week body weight, (C) 8-week body weight, (D) total food intake, (E) visceral adipose tissue weight. A: n = 5 rats per group. B-D: n = 6 rats per group. E: n = 4 rats per group. Independent-samples t-test was used for statistical analysis.
Fig 2.
Changes in lipids in the hypoxia group versus the normoxia group.
Lipids were classified according to the guidelines of the International Lipid Classification and Nomenclature committee. (A) Number of lipid classes and lipid species, (B) total lipid molecular content, and (C and D) content of different lipid subgroups. Independent-samples t-test was used for the statistical analysis of data presented in panels B, C, and D.
Fig 3.
Univariate and multivariate analyses of lipids in rat visceral adipose tissue.
(A) PCA score plot, (B) PLS-DA score plot, (C) PLS-DA permutation test, (D) OPLS-DA score plot, (E) OPLS-DA permutation test, and (F) volcano plot of the normoxia and hypoxia groups.Differences in lipid molecules with FC >1.5 or FC <0.67 and p-value <0.05 are represented by different colors. The rose-red dots in the figure represent significantly different lipids.
Fig 4.
Effects of hypoxia on markers of lipolysis.
Haematoxylin and eosin staining, scale bar:10μm, (B) adipocyte diameter, and (C) adipocyte area changes in the visceral adipose tissue in the hypoxia group versus the normoxia group. The mRNA levels of lipolysis related-genes,including (D) adipose TG lipase (ATGL), and (E) hormone-sensitive lipase (HSL). (F) The content of NEFA. A-C: n = 3 rats per group.D-E: n = 6 rats per group. Independent-samples t-test was used for statistical analysis.
Fig 5.
Analysis of significantly different lipids between the normoxia and hypoxia groups.
(A) DG carbon chain length analysis. (B) DG saturation analysis. (C) TG carbon chain length analysis. (D) TG saturation analysis. **p <0.01 versus the normoxia group; *p <0.05 versus the normoxia group.
Fig 6.
Clustering and correlation analyses of lipids in rat visceral adipose tissue.
Correlation clustering heat map. Red represents positive correlation and purple represents negative correlation. Color intensity is related to the absolute value of the correlation coefficient; i.e., the higher the positive or negative correlation, the darker the color. (B) Hierarchical clustering heat map.