Fig 1.
Growth of L. ferriphilum DSM 14647.
Curves represent the growth of adapted and exposed to 180 mM NaCl (grey circles), and non-adapted and non-exposed (white circles) cell cultures. Data represent the average of 3 independent experiments. Error bars represent standard deviation. Initial cell density, 1x106 cells mL-1.
Table 1.
Minimum inhibitory concentration of NaCl in L. ferriphilum DSM 14647 adapted to 180 mM NaCl at different external pH (pHex).
Table 2.
Up-regulated genes in L. ferriphilum DSM 14647 adapted to 180 mM NaCl in relation to non-adapted non-exposed control cells.
Table 3.
Down-regulated genes in L. ferriphilum DSM 14647 adapted to 180 mM NaCl in relation to non-adapted non-exposed control cells.
Fig 2.
Effect of NaCl adaptation on oxygen consumption rate in L. ferriphilum DSM 14647.
The measurements were carried out in adapted (A) cells exposed to 180 mM NaCl and non-adapted (NA) cells exposed to 0 or 180 mM NaCl. The data represent the average of 3 independent experiments. Error bars represent standard deviation. Statistical analysis was carried out by an ANOVA test.
Fig 3.
Effect of NaCl adaptation on the content of compatible solutes in L. ferriphilum DSM 14647.
Ectoine and hydroxyectoine content was measured in adapted cells exposed to 180 mM NaCl (A) and non-adapted (NA) cells exposed to 0 or 180 mM NaCl. The data represent the average of 3 independent experiments. Error bars represent standard deviation. Statistical analysis was carried out by ANOVA and a T Test. N.D.: not detected.
Fig 4.
Effect of NaCl on ROS generation in L. ferriphilum.
ROS were measured in adapted cells exposed to 180 mM NaCl (A) and non-adapted (NA) cells exposed to 0 or 180 mM NaCl. Cytoplasmic ROS content is expressed as relative fluorescence units (RFU) of the activated fluorescent probe H2DCFDA per mg of protein. The data represent the average of 3 independent experiments. Error bars represent standard deviation. Statistical analysis was carried out by ANOVA and a T Test.