Fig 1.
Process of LAMP method for nucleic acid detection extracted by paper-based microfluidics.
Fig 2.
Adsorption of DNA and H2O on paper-based microfluidics.
Notes: A, fluorescence of paper chip; B and C standard line of different concentration of DNA.
Table 1.
Comparison of paper chip nucleic acid extraction method and other methods.
Fig 3.
Specificity of LAMP detection with paper-based microfluidics extraction.
Notes: A1 & A2, LAMP amplification of peanut and soybean; B1 & B2, corresponding ladder electrophoresis bands of peanut and soybean.
Fig 4.
LAMP detection and electrophoresis verification based on the extraction DNA by paper-based microfluidics.
Notes: A1 & A2, LAMP amplification curves of peanut and soybean; B1 & B2, corresponding ladder electrophoresis bands of peanut and soybean.
Fig 5.
LAMP detection of different concentrations of DNA on paper-based microfluidics.
Notes: A1 & A2, amplification curves of peanut and soybean DNA with different concentrations; B1 & B2, linear relationship between peanut and soybean DNA concentrations and Ct value.
Fig 6.
Sensitivity of LAMP detection with paper-based microfluidics extraction.
Notes: A1, B1 & C1, the fluorescence amplification results, melting curve results, and agarose gel electrophoresis results of different peanut diluted samples; A2, B2 & C2, the fluorescence amplification results, melting curve results, and agarose gel electrophoresis results of different soybean diluted samples.
Fig 7.
Repeatability of LAMP detection with paper-based microfluidics extraction.
Notes: A1 & A2, the amplification results of peanut and soybean with 5 repeats; B1 & B2, Ct value of peanut and soybean with 5 repeats.
Fig 8.
Detection of peanut and soybean allergens in different areas and varieties by LAMP with paper-based microfluidics extraction.
Notes: 8A1 & 8C1, amplification curves appeared in peanut samples from different regions and varieties; 8B1 & 8D1, Tm values of peanut from different varieties and different places; 8A2 & 8C2, amplification curves appeared in soybean samples from different regions and varieties; 8B2 & 8D2, Tm values of soybean from different varieties and different places.
Fig 9.
Detection of peanut and soybean allergens in commercial products.
Notes: A1 & A2, LAMP amplification curves of different products containing peanut and soybean; B1 & B2, corresponding ladder electrophoresis bands o of different products containing peanut and soybean.
Table 2.
The detected Ct values and DNA concentration values of commercially available products in this method.