Fig 1.
A) Melanin pigment on peptone yeast extract iron agar medium by S. puniceus RHPR9. B). Production of melanin pigment on peptone yeast extract iron broth medium by S. puniceus RHPR9. C). Crystalline form of melanin pigment from S. puniceus RHPR9.
Fig 2.
UV-visible absorbance spectrum of purified melanin pigment of S. puniceus RHPR9.
Fig 3.
FTIR spectrum of melanin pigment of S. puniceus RHPR9.
Fig 4.
1HNMR Spectroscopy of the melanin pigment of S. puniceus RHPR9.
Fig 5.
Cell viability assays of HEK 293, HeLa, and SK-MEL-28 cell lines were tested for 24 h with various concentrations ranging from 0.5 to 100 μg/mL in 96 well plates.
The cell viability was determined at maximum absorption at 570 nm. A graph was depicted with melanin concentrations on the x-axis and percent viable cells on the y-axis.
Fig 6.
In-vitro wound-healing assay on HeLa cells: (0 h) wound was made by scratching a monolayer of HeLa cells with 100 μl sterile tip and treated with indicated concentrations of melanin.
To assess the degree of wound healing, cell migration towards the scratched areas was visualized and photographed at 0 h and then at 24 h.
Fig 7.
The data was calculated from two independent where random fields were selected and quantified using ImageJ software.
Table 1.
Antibacterial activity against pathogenic bacteria.