Fig 1.
Schematic representation of library preparation and NGS.
Data for D3S1358 is shown as a representative example. (a) Amplification of the target sequence with barcoding primers. (b) Second PCR amplification adding specialized adapters and indexes to both ends. (c) Purification of the amplicon and pooling multiple libraries together. (d) Linearization of DNA libraries. (e) Loading DNA libraries onto the flow cell. (f) Bridge PCR amplification. (g) Cluster generation. (h) Paired-end sequencing. (i) Base calling, alignment and data analysis.
Fig 2.
Flow chart for experimental procedures and data analysis.
Fig 3.
The NGS sequencing reads per sample per locus of plasma DNA (range: 1068–140045 reads).
Fig 4.
Overview of target sequence length for all STR loci as per NGS and CE.
Target sequence indicates the PCR amplicon without barcoding and adapter primers.
Table 1.
The paternity testing parameters using cffDNA in the 14 cases with male fetuses.