Fig 1.
Effect of CXCL1 on the proliferation and migration of CCA cells.
A, CXCR2 expression on OCUG-1 cells and HuCCT1 cells. B, CXCL1 significantly inhibited the proliferative potential of OCUG-1 cells and HuCCT1 cells at 72 h. **, p<0.01 vs control. C, CXCL1 significantly inhibited the migration potential of OCUG-1 cells after 24 h and HuCCT1 cells after 12 h. **, p<0.01 vs control.
Fig 2.
Representative immunostaining images of CXCL1 and CXCR2 expression.
A, CXCL1 was expressed in CCA cells. CXCR2 was observed at both the cell membrane and the cytoplasm. Intensity score: 0, negative; 1+, weakly positive; 2+, positive; 3+. Bar: 100 μm. B, CXCL1 and CXCR2 expression in a case. Bar: 100 μm.
Table 1.
The correlation between the clinicopathologic features and CXCL1 and/or CXCR2 in CCAs.
Fig 3.
The overall survival rate of CCA patients according to CXCR2 expression.
The patients with a positive expression of CXCR2 (n = 29) had a significantly better survival rate than those without CXCR2 expression (n = 132). The patients with positive expressions of both CXCL1 and CXCR2 (n = 28) had a significantly better survival rate than those with no CXCR2 expression (n = 132), but the patients with positive CXCL1 expression (n = 121) did not have a significantly better survival rate than those without CXCL1 expression (n = 44).
Table 2.
Univariate and multivariate analysis with respect to overall survival.