Table 1.
List of primer pairs used in the study.
Fig 1.
Phylogenetic tree of B. tabaci cryptic species identified based on cytochrome oxidase subunit I (COI) sequences.
The samples from the study are indicated by bold text in the tree; all other sequences were obtained from the GenBank database. Pink and blue coloured shade represent sequences of Asia I and Asia II 5 respectively, whereas, yellow shade represent the other cryptic species of B.tabaci. Bemisia afer sequences (green shade) was taken as an out-group.
Fig 2.
Real-time PCR analysis of YVMV indicate the specificity of the reactions (A) Thermal profile (B) PCR amplification plots (C) dissociation curve and (D) standard curves of serially diluted linearized plasmids obtained using SYBR® Green chemistry. The specific melting temperature for YVMV products was around 78°C. Standard curves show a linear relationship between initial viral copy number per microliter on X-axis and Ct values on Y-axis. Each concentration was replicated twice. The equation of the straight line and the coefficient of correlation (R2) are mentioned on the graph.
Fig 3.
Real-time PCR analysis of OELCuV indicate the specificity of the reactions (A) Thermal profile (B) PCR amplification plots (C) dissociation curve and (D) standard curves of serially diluted linearized plasmids obtained using SYBR® Green chemistry. The specific melting temperature for YVMV products was around 84°C. Standard curves show a linear relationship between initial viral copy number per microliter on X-axis and Ct values on Y-axis. Each concentration was replicated twice. The equation of the straight line and the coefficient of correlation (R2) are mentioned on the graph.
Fig 4.
Dynamics of YVMV and OELCuV copies ingested (A and B) and egested (C and D) by individual Asia I and Asia II 5 genetic group of B. tabaci. Acquisition/ Inoculation feeding time is expressed on X-axis and log of virus copy number is plotted on Y-axis. YVMV and OELCuV copies were estimated at 1 min, 5 min, 15 min, 1 hr, 2 hrs, 6 hrs, 12 hrs, and 24 hrs of feeding. The different letters indicate statistically significant differences between the treatments. The bars represent the standard error of mean (± SEM).
Table 2.
Copies of YVMV and OELCV ingested by individual B. tabaci (Asia I and Asia II 5) genetic group at different feeding exposure.
Table 3.
Copies of YVMV and OELCV egested by individual B. tabaci (Asia I and Asia II 5) genetic group at different feeding exposure.