Fig 1.
Sampling localities for 327 Eastern Massasauga collected from nine hibernacula across three study areas at Carlyle Lake, Illinois, USA. All map data (road layer, municipal boundaries, streams, lakes, and protected lands) were publicly accessible and downloaded from the Illinois Geospatial Data Clearinghouse [36] and from I-View [37] and then projected in ARCMAP 10.8.0 [38].
Table 1.
Standard genetic diversity indices for Eastern Massasauga Rattlesnake at Carlyle Lake.
Fig 2.
Bayesian clustering results for 327 Eastern Massasauga sampled from nine hibernacula across three study areas at Carlyle Lake, Illinois.
Structure analyses revealed K = 3 distinct genetic clusters that corresponded to the three main study areas; Dam Recreation Area, Eldon Hazlet State Park, and South Shore State Park. Data were derived from 21 microsatellite loci, and full names of sampling locations are provided in Table 1.
Fig 3.
Bayesian clustering results for 115 Eastern Massasauga sampled from six hibernacula within Eldon Hazlet State Park at Carlyle Lake, Illinois.
Structure analyses revealed K = 1–3 optimal genetic clusters. Data were derived from 21 microsatellite loci, and full names of sampling locations are provided in Table 1.
Table 2.
Pairwise estimates of FST (below diagonal) and G”st (above diagonal) for 327 Eastern Massasauga sampled from nine hibernacula across three study areas as at Carlyle Lake, Illinois.
Table 3.
Pairwise estimates of FST (below diagonal) and G”st (above diagonal) for Eastern Massasauga sampled from the three main study areas at Carlyle Lake, Illinois.
Table 4.
Analysis of Molecular Variance (AMOVA) for 327 Eastern Massasauga sampled from nine hibernacula across three study areas at Carlyle Lake, Illinois.
Table 5.
Estimates of K values (genetic clusters) across all hibernacula.
Table 6.
Estimates of K values (genetic clusters) across Eldon Hazlet State Park (EHSP) hibernacula.
Fig 4.
Migration estimates for 327 Eastern Massasauga sampled from nine hibernacula across three study areas at Carlyle Lake, Illinois.
Structure analyses revealed K = 3 distinct genetic clusters that corresponded to the three main study areas; Dam Recreation Area (DMRA), Eldon Hazlet State Park (EHSP), and South Shore State Park (SSSP). Arrows represent estimates of asymmetric migration rates (±standard error) among the three genetic clusters, and circles represent estimates of the proportion of non-migrant individuals in each cluster. Data were derived from 21 microsatellite loci, and full names of sampling locations are provided in Table 1.
Table 7.
Estimates of effective population size (Ne) for Eastern Massasauga sampled from Carlyle Lake, Illinois.