Fig 1.
A Circos plot showing the distribution of C. jejuni LOS locus classes (A-W), subclasses (A1, A2, B1, B2) and LOS groups (1–4) in the online C. jejuni sequence database.
Each segment of the inner circle specifies the total number of C. jejuni strains sequences (703) extracted for the LOS classification. The frequency of C. jejuni isolates classified for each particular LOS class/group is mentioned in numbers (n out of 703) on the top of each inner circle segment and represented with ribbon width. The frequency of a C. jejuni LOS class/group in percentage (% of overall frequency-100) is shown with each outer circle segment and represented by the orange or coloured bars. Ribbons link each C. jejuni LOS class to its related LOS group.
Fig 2.
A Circos plot showing the distribution of C. jejuni LOS locus classes (A-W), subclasses (A1, A2, B1, B2) and LOS groups (1–4) from clinical isolates.
Each segment of the inner circle specifies the total number of classified C. jejuni isolates (50) used for the PCR based typing assay. The frequency of C. jejuni isolates classified for each particular LOS class/group is mentioned in numbers (n out of 50) on the top of each inner circle segment and represented with ribbon width. The frequency of a C. jejuni LOS class/group in percentage (% of overall frequency-100) is mentioned with each outer circle segment and represented by the orange or coloured bars. Ribbon ends link each C. jejuni LOS class to its related LOS group.
Fig 3.
A comparison of C. jejuni LOS biosynthesis locus class and group frequencies found in the collections of GenBank C. jejuni sequences (n = 703) and C. jejuni clinical, typed strains (n = 50).
Fig 4.
The genetic organisation of C. jejuni 1336, C. jejuni 414, and C. jejuni CFSAN05410 that contain novel LOS gene content.
13 LOS biosynthesis genes in C. jejuni 1336, 5 LOS biosynthesis genes in C. jejuni 414 and 5 LOS biosynthesis genes in C. jejuni CFSAN054107 occurred between previously known LOS genes (cj1135, ORF17, and waaV). Green arrows: previously reported LOS genes in C. jejuni strains; Pink arrows: previously known, variable LOS genes that had similarity to the LOS biosynthesis genes of other C. jejuni strains; Blue arrows: LOS genes that had similarity to the CPS biosynthesis genes of other C. jejuni strains; Purple arrows: LOS genes that had similarity to the LOS biosynthesis genes of C. coli strains. The direction of arrow represents the direction of gene transcription. Black star: Gene with an unknown function.
Fig 5.
Distribution of C. coli LOS locus classes within the online C. coli sequences GenBank database.
The number of C. coli strains associated with each C. coli LOS class in 2D column chart and corresponding percentages of C. coli strains in Pie chart, represent the frequency of C. coli LOS locus classes within the global collection of C. coli isolates.
Fig 6.
Genomic organisation of previously unreported C. coli LOS core genes and their relative similarity.
The position of novel gene content between waaF and gmhA in C. coli LOS types (I, II, III, V and VIII). The sizes of these genes, obtained from the GenBank database, are given in kb. Each dotted line links two genes to represent the similarity between them in terms of query cover score (non-bold) and Megablast identity score (bold).
Fig 7.
Frequency of C. jejuni LOS locus classes in different Campylobacter sources.
Environment involves animal farm soil and animal farm water as Campylobacter sources.
Fig 8.
Frequency of C. coli LOS locus classes in different Campylobacter sources.
Environment involves animal farm soil and animal farm water as Campylobacter sources.