Table 1.
Comparative analysis of spectrometric data from six DNA extraction methods.
Fig 1.
Comparison of six methods of Loa loa DNA extraction.
Extracted DNA in duplicate was analyzed by electrophoresis in a 0.8% agarose gel and visualized under UV light. PM = standard molecular weight. Lines 1–2: DNA from phenol/chloroform; 3–4: Qiagen extract; 5–6: Salting-out extract; 7–8: Tris-EDTA extract; 9–10: Methanol extract; 11–12: CTAB extract. The values on the left are the size of molecules.
Fig 2.
Digestion of extracted Loa loa DNA by EcoRI and BamHI endonuclease.
2A: Undigested (lines: 1-3-5-7-9-11) compared to digested with EcoRI (lines: 2-4-6-8-10-12) DNA; extracts were analyzed via agarose gel electrophoresis (1%) and visualized under UV light. The values on the left indicate the size of the bands. PM = molecular marker. 2B: Undigested (lines:1-3-5-7-9-11) compared to digested with BamHI (lines:2-4-6-8-10-12) DNA; extracts were analyzed via agarose gel electrophoresis (1%) and visualized under UV light. The values on the left indicate the size of the bands. PM = molecular marker.
Fig 3.
Amplification of DNA extracted by PCR.
Comparison of amplicon obtained after amplification of Loa loa DNA extracted with the six methods as template. The primers were designed from the Brugia ALT1 gene. Results of the analysis of amplicons after agarose gel electrophoresis (1.5%) and visualization under UV light. The methods are listed on the top of each band: phenol:chlorof line 1; Qiagen line 2; salting out line 3; Tris-EDTA line 4; Methanol line 5; CTAB line 6; band 7 is a negative control. PM = molecular marker. The values on the left represent the size of DNA.