Fig 1.
Diagram showing the process of selection of the 1103 sheep tested in the study.
Fig 2.
The location of the starting 120 sheep flocks categorized according to their anti-Fasciola antibody status is shown. The flocks finally selected for the study are also displayed. Reprinted from BDLJE under a CC BY license, with permission from ign.es, original copyright 2015.
Fig 3.
Distribution of OD values obtained with eMM3-COPRO test in the ‘Fasciola-free’ sheep population (reference population).
The solid vertical red line indicates the 99th percentile value (OD = 0.013) and dotted vertical lines indicate the lower (OD = 0.009) and upper (OD = 0.015) limits of the confidence interval for that percentile (P = 0.01). The reference population included 561 sheep from 15 flocks.
Table 1.
Helminths and protozoa identified in the population of ‘Fasciola-free’ sheep.
Table 2.
Precision profile of the eMM3-COPRO test.
Table 3.
Results obtained by analyzing the faecal samples collected in the ‘Fasciola-infected’ flocks.
Fig 4.
Effect of treatment with triclabendazole on the OD values of sheep testing positive by eMM3-COPRO test.
The dose of triclabendazole was 10 mg/kg. Analyses were performed immediately before drug administration and 3 weeks post-treatment. Results are shown separately for sheep with negative (A) and positive (B) coprology. Dashed red line indicates the cut-off value (0.021).
Fig 5.
OD values of the ‘Fasciola-infected’ flocks’ sheep that tested negative in both eMM3-COPRO and coproscopy.
(A) Scatter plot showing the distribution of individual values and (B) diagram of frequencies (n = 282). Dashed red lines indicate the cut-off value (0.021). Dashed green lines indicate the maximum OD value (0.017) in the negative reference population. Solid blue lines indicate the separation between samples from different flocks (1–13).
Fig 6.
Individual optical density values for the sheep diagnosed as positive by eMM3-COPRO test.
Data (n = 260) were clustered into 5 categories based on the faecal egg count. For each category, the median values are represented by a solid horizontal red line. The dashed red line indicates the cut-off value (0.021).
Fig 7.
Operational characteristics of eMM3-COPRO test versus coproscopy.