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Fig 1.

PCoA analysis of frozen and FFPE fetal membrane samples and processed negative controls.

PCoA plots analysed from V4 16S rRNA amplicon sequencing of DNA extracted from Formalin-Fixed Paraffin-Embedded (FFPE) fetal membrane samples and frozen stored fetal membrane tissues (A). FFPE samples compared to corresponding surrounding paraffin wax negative controls (B), and additionally FFPE samples compared to DNA extraction kit reagent negative controls (C).

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Fig 1 Expand

Fig 2.

Genera with significantly different read counts between FFPE and frozen fetal membrane samples.

Plots created for nine genera which were detected as significantly different via univariate analysis between Formalin-Fixed Paraffin-Embedded (FFPE) fetal membrane samples and frozen stored fetal membrane tissues. Data presented by read count and log transformed.

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Fig 2 Expand

Table 1.

Read count comparison between sample type and contamination controls.

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Table 1 Expand

Fig 3.

Genera with significantly different read counts between FFPE samples and DNA extraction kit reagent negative controls.

Plots created for 21 genera which were detected as significantly greater in Formalin-Fixed Paraffin-Embedded (FFPE) fetal membrane samples compared DNA extraction kit reagent negative controls via univariate analysis. Data presented by read count and log transformed.

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Fig 3 Expand

Fig 4.

Genera with significantly greater read counts in DNA extraction kit reagent negative controls compared to FFPE samples.

Plots created for four genera which were detected as significantly greater in DNA extraction kit reagent negative controls compared to Formalin-Fixed Paraffin-Embedded (FFPE) fetal membrane samples via univariate analysis. Data presented by read count and log transformed.

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Fig 4 Expand

Table 2.

Decontam output classified into true or contaminating genera.

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Table 2 Expand

Fig 5.

Percentage contribution for bacterial source in Formalin-Fixed Paraffin-Embedded (FFPE) fetal membrane samples.

SourceTracker results displayed as a pie chart per individual patient sample. Source origins include DNA extraction reagents (Kit Neg), paraffin wax surrounding the tissue sample (Wax Neg), and source of unknown origin (Unknown).

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Fig 5 Expand

Table 3.

Proportion contribution to determine origin source of microbial composition in samples.

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Table 3 Expand