Table 1.
Enrichment analysis of chloroplast proteins in Eucalyptus grandis using a comparative approach between total leaf protein extract and chloroplast protein extract.
Fig 1.
The Eucalyptus grandis chloroplast proteome.
Classification analysis, in terms of the number of identified proteins (A) and terms of the percentage of identified proteins (B), upon categorization using the “biological process” ontology; protein expression analysis of the Gene Ontology terms (C) and number of protein identifications per chloroplast sub-cellular compartment (D).
Fig 2.
Multivariate analyses of the 61 differentially abundant chloroplast proteins isolated from young, middle, and mature Eucalyptus grandis leaves.
Principal component analysis (A), clustering expression analysis (B) and functional categorization of the differentially-regulated chloroplast proteins between young (downregulated) and mature (upregulated) (C). The z-score indicates the distance of the data compared to the mean value.
Fig 3.
Expression trends of the differentially regulated chloroplast proteins.
Upregulated proteins increased abundance throughout leaf development, while downregulated proteins decreased abundance during leaf development.
Fig 4.
Meteorological data recorded at the Sao Paulo State University Forest Nursery during Eucalyptus grandis cultivation.
Changes in temperature and rainfall rates between August 2019 and September 2020 (A) and multivariate analysis of the chloroplast proteome samples collected in the summer, fall, winter, and spring (B).
Fig 5.
Differentially abundant proteins identified in Eucalyptus grandis chloroplasts in different seasons of the year.
Hierarchical clustering (A) and protein abundance trends (B). The z-score indicates the distance of the data compared to the mean value.
Fig 6.
Most differentially abundant chloroplast proteins isolated from Eucalyptus grandis leaves in studies seasons.
Red boxes indicate proteins with significant change higher than 2-folds. Blue boxes indicate a significant change higher than 5-folds. White boxes indicate proteins with change lower than 2-folds.