Fig 1.
The samples were obtained in the states of Yucatan (purple), Tabasco (orange), Chiapas (green), Queretaro (brown), San Luis Potosi (yellow) and Sinaloa (light green).
Table 1.
Geographical and climatic characteristics of the sampling locations.
Fig 2.
Ticks from Tabasco and San Luis Potosi (pink). Ticks from Queretaro (blue) and ticks from Yucatan (red). No ticks were found on equines from Sinaloa and Chiapas (grey).
Fig 3.
Positive samples to T. equi (blue bars). Positive samples to B. caballi (orange bars). Negative samples (grey bars). Coexposed (yellow bars). The table section shows a summary of the results.
Fig 4.
Antibody detection percentages for cELISA.
Positive samples to T. equi (orange). Positive samples to B. caballi (yellow). Double-negative (green). Double-positive (brown).
Fig 5.
Positive samples to T. equi (blue bars). Positive samples to B. caballi (orange bars). Negative samples (grey bars). Coinfected samples (yellow bars). The table section shows a summary of the results.
Fig 6.
Detection percentages for nPCR.
Positive samples to T. equi (orange), positive samples to B. caballi (yellow). Double-negative (green). Double-positive (brown).
Fig 7.
Kappa statistic calculations for the test results generated by the molecular assays used in this study.
A) 2×2 contingency table for T. equi. B) 2×2 contingency table for B. caballi. C) Infection prevalences calculated using the nPCR and duplex qPCR test results.
Fig 8.
Phylogenetic tree constructed using RAP1 amino acid sequences generated in this study and similar sequences deposited in GenBank (sequence names start with an accession number).
Translated Z49818 sequence of the B. divergens rap1 gene served as an outgroup. Bootstrap support values over 50 are shown on the nodes.
Fig 9.
Phylogenetic tree constructed using EMA1 amino acid sequences generated in this study and similar sequences deposited in GenBank (sequence names start with an accession number).
Translated TMU85039 sequence of the T. mutans homologue served as an outgroup. Bootstrap support values over 50 are shown on the nodes.