Fig 1.
The sampling cities of rodents in Hainan Province from 2016 to 2019.
A: The map of China. B: The sampling locations in 2016 including Dongfang and Wenchang. C: The sampling locations in 2017 including Dongfang, Danzhou, Haikou and Wenchang. D: The sampling locations in 2018 including Dongfang and Sansha. E: The sampling locations in 2019 including Sansha. Note: The map source referred to maps at the CIA which was similar but not identical to the original image and was therefore for illustrative purposes only.
Fig 2.
The positive rate in different cities during 2016–2019.
F: The positive rate of hantavirus in Hainan Province in 2016. G: The positive rate of hantavirus in Hainan Province in 2017. H: The positive rate of hantavirus in Hainan Province in 2018. I: The positive rate of hantavirus in Hainan Province in 2019.
Table 1.
Detection of hantavirus in rodents in Hainan Province from 2016 to 2019.
Table 2.
Analysis of the nucleotide and amino acid similarity of hantavirus L segment in Hainan Province and surrounding areas.
Table 3.
Analysis of the nucleotide and amino acid similarity of hantavirus M segment in Hainan Province and surrounding areas.
Table 4.
Analysis of the nucleotide and amino acid similarity of hantavirus S segment in Hainan Province and surrounding areas.
Table 5.
Sequence analysis of Hantavirus in Hainan with other genotypes and subtypes.
Fig 3.
The phylogenetic tree based on ORF of L segment nucleotide sequences of hantavirus in Hainan and others.
According to the phylogenetic analysis, the “L” sequences of the four hantavirus strains sequences found in Hainan Province belong to S2 subtype of SEOV. Note: The small red triangle represents the newly discovered hantavirus sequences in this study.
Fig 4.
The phylogenetic tree based on ORF of M segment nucleotide sequences of hantavirus in Hainan and others.
The ‘M’ sequences (named HN1M and HN4M) of the two hantavirus HN1 and HN4 show a close genetic distance with GZRn76, HN71-L, Guang199 and Beijing-Rn, which belong to S2 subtype. Note: The small red triangle represents the newly discovered hantavirus sequences in this study.
Fig 5.
The phylogenetic tree based on ORF of S segment of hantavirus nucleotide sequences in Hainan and others.
The HN1 and HN4 are located in the same branch of the phylogenetic tree with GZRn76, GZRn148 and GuangzhouRn36 isolated from Guangzhou. Note: The small red triangle represents the newly discovered hantavirus sequences in this study.
Table 6.
The amino acid variation analysis between HN1 and HN4 and SEOV 80–39.
Fig 6.
The tertiary structure of glycoprotein based on M segment.
A: The amino acid variation analysis between HN1 and Z37 and L99 with the highest similarity to GC protein. B: The amino acid variation analysis between HN4 and Z37 and L99 with the highest similarity to GC protein.
Table 7.
The amino acid variation analysis between HN1 and HN4 and Z37 and L99.