Table 1.
Summary of raw tags after filtering and mapping to the reference genome of each library.
Fig 1.
The volcano plots of differentially expressed genes between Hu sheep (HZ) and Tibetan sheep (ZZ) in fat tail.
The x-axis shows the values of log2 (fold change), while the average expression values of -log10 (p) are displayed by the y-axis. The blue and red dots represent the significantly differentially expressed transcripts (p < 0.05) comparing the HZ and ZZ, with blue for downregulated genes and red for upregulated genes. The black dots indicate the transcripts with expression levels which are not statistically significant (p > 0.05) comparing HZ and ZZ.
Fig 2.
Venn diagram of targeting relationship of DE miRNAs.
The number is the intersection of the relationship pairs predicted by RNAhybrid and miRanda.
Fig 3.
Target gene function enrichment of GO and KEGG.
(A) Each top ten enrichment pathways for BP, CC, MF of target genes. The x-axis displays enrichment, and the y-axis represents the GO terms. The filled colored circles display each statistically significant GO term. The size of circles represents gene number. (B) Top sixty pathways of target genes. The x-axis displays rich factor of target genes, and the y-axis represents the KEGG pathway. The filled colored circles represent each statistically significant KEGG pathway. The filled colored circles display each statistically significant KEGG term. The size of circles represents gene number.
Fig 4.
(A) RNA-Seq and RT-qPCR results of ten selected differentially expressed miRNAs in HZ and ZZ. (B) RT-qPCR results of HOXC9 in HZ and ZZ. * indicates significant difference (P<0.05).
Fig 5.
Result of the luciferase reporter assay.
(A) Potential binding site between oar-miR-379-5p and HOXC93’UTR. The underlined sequences represent the mutant sites. (B) WT display the psiCHECK2-HOXC9-3′UTR-WT. MT display psiCHECK2-HOXC9-3′UTR-MT. psiCHECK2 display psiCHECK2 pure vectors. Mimics display miR-379-5p mimics. ** indicates highly significant difference (P<0.01).