Fig 1.
Results of correlation analysis.
(A) Association between stromal score and CRC AJCC stage. (B) Association between the immune score and CRC AJCC stage. (C) Correlation between the stromal score group and the survival time of patients with CRC. (D) Correlation between the immune score group and the survival time of patients with CRC.
Fig 2.
Estimate score C5 enrichment analysis results based on the ESTIMATE algorithm.
(A) GSEA of top one pathway enriched for high stromal group (NES = 2.54, nominal P < 0.0001). (B) The top5 significant biological pathways in high stromal score group (sort by NES). (C) The enrichment plot of amide binding (NES = 2.61, nominal P < 0.0001). (D)The top5 significant biological pathways in high immune score group (sort by NES).
Fig 3.
The expression profiles and differential expression analysis results of the top 50 characteristic genes between the high and low stromal and immune score groups.
(A) The expression heat map for the top 50 DEGs based on immune score grouping. (B) The expression heatmap for the top 50 DEGs based on stromal score grouping. (C) Volcano maps based on differential expression analysis of immune score groups. (D) Volcano maps based on differential expression analysis of stromal score groups.
Fig 4.
Network pharmacology analysis of SJZD.
(A) Drug-active ingredient-target network diagram. The blue nodes are drug targets, and the red nodes are compounds of SJZD. (B) Intersection of 230 therapeutic targets and 1557 DEGs related to SJZD. (C) PPI network diagram of the 52 core genes. The larger nodes in the inner ring represented the most important hub nodes. The smaller nodes in the outer ring represent the other nodes (several nodes with degrees of 1 were deleted).
Table 1.
Basic information for some active components of SJZD.
Table 2.
A partial information table for the core targets.
Fig 5.
GO (BP, MF, CC) enrichment analysis and KEGG pathway enrichment analysis of 52 nodes.
Fig 6.
Survival analysis results for the 52 core target genes (performed in 214 CRC samples from GEO).
Fig 7.
Verification results of survival analysis (performed in 530 COAD samples from TCGA).