Table 1.
Demographic characteristics of the study population.
Fig 1.
Comparison of DNA quality and bacterial diversity using two DNA isolation kits (Ct and Qia).
A. DNA yield. The gray lines represent the same sample pair. B. DNA purity. C. OTUs D. Chao1 index.
Fig 2.
Relative abundance of microbiome of adults and children.
A. Variation in bacterial relative abundance at the phylum level with Ct kit. B. Bacterial relative abundance at the phylum level with Qia kit. C. Bacterial relative abundance at the genus level with Ct kit. D. Bacterial relative abundance at the genus level with Qia kit.
Fig 3.
Comparison of relative abundance of four major microbiomes at phylum level using Ct and Qia kits.
G+: Gram-positive bacteria. G-: Gram-negative bacteria.
Fig 4.
Firmicutes/Bacteroidetes (F/B) ratio of subgroups.
A. F/B ratio distribution between normal vs. obese group. B. F/B ratio distribution between adult vs. children group. C. F/B ratio distribution between Ct and Qia kits in Normal and obese groups, respectively. The gray lines represent the same sample pair. D. F/B ratio distribution between Ct and Qia kits in adults and children groups, respectively.
Fig 5.
Beta diversity observation in different subgroups.
A. NMDS plot of normal and obese group microbiome communities with Ct kit. B. NMDS plot of adults and children microbiome communities with Ct kit. C. NMDS plot of normal and obese group microbiome communities with Qia kit. D. NMDS plot of adults and children microbiome communities with Qia kit.
Table 2.
List of taxa showing different abundance between adults and children at the genus level.
Fig 6.
Characterization of microbiomes of adults and children by LEfSe and LDA analysis.
A. Taxa with significant differences in abundance between adults and children with Ct kit. B. Taxa with significant differences in abundance between adults and children with Qia kit. Histogram of log10(LDA scores) for features with differential abundance between adults and children with Ct kit (C) and with Qia kit (D). Taxa of |log10(LDA scores)| >3 are presented.