Table 1.
Soil samples taken from different locations of Lucknow, UP, India.
Fig 1.
Images showing clear zone of diameter of microbial colonies in (A) and (B). Positive colonies showing PHB hydrolysis on PHB agar media (C) CA6-(55), (D) CB2-(20), and (E) Kuk1-(34).
Table 2.
Enzyme activity and CFU count of positive bacterial isolates.
Fig 2.
Phylogenetic tree of Aeromonas caviae Kuk1-(34) sp. drawn using UPGMA tree (MEGA X software) with the evolutionary distances showing the relationship of PHB depolymerase producing bacteria with the known sequences of related genera.
Table 3.
Morphological, biochemical and physiological characteristics of the bacterial strain Kuk1-(34).
Table 4.
16S rRNA identification of positive isolates.
Fig 3.
Degradation of PHB film treated with (A) control and Aeromonas caviae Kuk1-(34) sp. after (B) 15 days (C) 30 days and (D) 45 daysin soil burial method.
Table 5.
Weight loss degradability of PHB film after different incubation time in un-optimised conditions.
Fig 4.
Morphological changes in SEM micrographs of PHB film degraded in soil burial method after incubation with (A) Control PHB film without treatment, and Aeromonas caviae Kuk1-(34) sp after (B) 15 days (C) 30 days (D) 45 days.
Fig 5.
FTIR chromatogram of soil buried PHB film after the degradation by Aeromonas caviae Kuk1-(34) sp.
The functional groups changes were compared with each film after incubation with (A) Control PHB film without treatment, Aeromonas caviae Kuk1-(34) sp after (B) 15 days (C) 30 days (D) 45 days.
Fig 6.
Pareto chart of the significant factor in the (A) Plackett Burman (PB) design, (B) Central composite design (CCD) for the enzyme production,(C) Surface plot of enzyme activity of the bacterial strain Aeromonas caviae Kuk1-(34) sp., (D) Contour plot showing the interaction effect of temperature and time on PHB depolymerase enzyme production by Aeromonas caviae Kuk1-(34) sp.
Table 6.
Component design of Plackett Burman for PHB depolymerase production with the bacterial strain Aeromonas caviae Kuk1-(34) sp.
Table 7.
Placket Burman experimental design for PHB depolymerase production with the bacterial strain Aeromonas caviae Kuk1-(34) sp.
Table 8.
ANOVA analysis for Plackett Burman design for PHB depolymerase enzyme production.
Table 9.
Component design of CCD for PHB depolymerase production with the bacterial strain Aeromonas caviae Kuk1-(34) sp.
Table 10.
Central Composite Design for PHB depolymerase production with the bacterial strain Aeromonas caviae Kuk1-(34) sp.
Table 11.
ANOVA analysis of CCD for PHB depolymerase enzyme production.
Fig 7.
Soil burial method for the degradation of PHB film as compared with (A) control after treatment with the Aeromonas caviae Kuk1-(34) sp. for (B) 15 days (C) 30 days and (D) 45 days.
Table 12.
Weight loss degradability of PHB film after different incubation time in optimised conditions.