Table 1.
Primer and probe sequences for EGFR mutation detection.
Fig 1.
Droplet digital PCR probe design to detect EGFR L858R substitution (A) and EGFR exon 19 deletion (B).
Fig 2.
Droplet digital PCR test for EGFR L858R for wild type DNA (A) and mutant EGFR L858R DNA at 1% variant allele fraction (B). EGFR exon 19 deletion analysis for wild type DNA (C) and mutant EGFR E746_A750del at 1% variant allele fraction (D).
Fig 3.
Relationship between variant allele fraction (VAF), DNA loading concentration, and positive mutation events for EGFR L858R (A) and EGFR exon 19 deletion (B).
Fig 4.
Linear correlation between input DNA concentration and experimentally calculated concentration for EGFR L858R reaction (A) and EGFR exon 19 deletion reaction (B).
Table 2.
Number of positive mutant events for EGFR L858R reactions.
Table 3.
Number of positive mutant events for EGFR exon 19 deletion reactions.
Fig 5.
Experimental versus input variant allele fraction for specimens above limit of detection for EGFR L858R (A) and EGFR exon 19 deletion (B). Mean percent error decreases with increasing variant allele fraction for EGFR L858R (C) and EGFR exon 19 deletion (D). Coefficient of variation with respect to variant allele fraction for EGFR L858R (E) and EGFR exon 19 deletion (F).
Fig 6.
Histogram of turnaround times for droplet digital PCR compared to panel next generation sequencing.