Table 1.
Morphology descriptors used in this study.
Modified from PlantScreen User Manual, v1.5, 2017.
Fig 1.
Workflow used in this study for phenotyping and QTL mapping.
485 RILs (3 replicates) were grown in 5x4 trays and imaged daily. Images were automatically segmented, rosettes were extracted, and analysed by device built-in pipelines. Shape descriptors and other metadata are recorded into a database. Shape descriptors and their PCA-derived morphospace were used for QTL mapping with happy.hbrem followed by gene search at ARAPORT11.
Fig 2.
Rosette morphology variation of the MAGIC RIL population across time.
A. Top view images of two contrasting rosettes, RIL 41 and RIL 516. B. Projected rosette area (PRA) and compactness from a set of six different RILs. DAS: Days after sowing. Error bars represent sample-based standard deviation (SD, n = 3). The six MAGIC lines have been chosen so they cover most of the range of phenotypic variation.
Table 2.
Broad-sense heritability per morphology descriptor across time, showing the percentage of change between the last and first DAS.
Fig 3.
LOD score plots at different DAS.
A. Roundness. B. Compactness. C. Close-up of chromosome 2 region surrounding PHYB_1645 SNP (blue asterisk) and ER_472 SNP (green asterisk). Gene models are shown under marker names. Significance threshold–log(P) ≥ 3.5 is shown as dotted lines.
Table 3.
QTLs found per day after removing redundant QTLs.
Table 4.
QTLs found per variable after removing redundant QTLs.
Organized by shape descriptor, sorted by QTL count.
Table 5.
QTLs found per chromosome after removing redundant QTLs.