Fig 1.
PCR amplification curves for positive case 1 (A) and positive case 2 (B). Upper panel, N1; middle panel, N2; lower panel, internal control (IC) gene. Left column of each panel, 10-sample pools; right column of each panel, individual samples.
Table 1.
Individual amplification of 10 samples from the pool including positive case 1 (#8).
Table 2.
Individual amplification of 10 samples from the pool including positive case 2 (#20).
Fig 2.
Examples of good and poor PCR amplification.
(A) Good amplification with a sigmoid pattern. (B) Poor amplification lacking a sigmoid pattern.
Table 3.
Pooled and individual testing.
Fig 3.
Characteristics of survey participants.
(A) Age group distribution with males (blue) and females (light blue). (B) Locations at the time of screening, showing prefectures with more than 10 participants. Plots were generated using the survey result (S2 Table), R (version 4.1.1) and an open-source R package ‘NipponMap’ (https://CRAN.R-project.org/package=NipponMap).
Fig 4.
Responses to the online survey.
Plots were generated using the survey result (S2 Table).