Fig 1.
Host species differences in microbiota structure.
a) Inverse Simpson index estimates for individual samples of Daphnia magna and D. dentifera, with t-test results reported. b) Genus-level bacterial microbiota composition for D. magna and D. dentifera. Amplicon sequence variants (ASVs) identified at < = 1% of relative abundance in each host species and ASVs not able to be identified at the Genus level are denoted by “< = 1% Abundant/Unidentified”. c) Principal coordinate analysis using weighted UniFrac distances comparing D. magna and D. dentifera, with PERMANOVA results reported. Ellipses represent the multivariate t-distribution of D. magna and D. dentifera samples.
Fig 2.
Differences in microbiota structure at different times of day.
(a) Alpha diversity, represented by the inverse Simpson index, of Daphnia magna and D. dentifera during the day and night sample timepoints. For each species t-test results are reported. (b) Size-corrected feeding rates of D. magna and D. dentifera during the day and night sample time points (n = 14 per species). For each species t-test results are reported. (c) Genus-level composition plots for each sample taken for D. magna and D. dentifera at each timepoint (D. magna, n = 10; D. dentifera, n = 9).
Fig 3.
Comparisons of ASVs with more than 0.5% abundance across all samples by host species and time point.
Significance of t-tests for each ASV are denoted on the right side of each figure (*, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001). (a) ASVs found only in Daphnia magna in day (pink) or night (red) samples. ASV taxonomic identity is denoted at the most specific taxonomic rank possible without assigning species-level identity. (b) ASVs found only in D. dentifera in day (green) or night (blue) samples, with taxonomic identity as in (a). (c) & (d) ASVs common to both host species. Relative abundances of ASVs in D. magna in (c) and relative abundances of ASVs in D. dentifera in (d), with taxonomic identity as in (a).