Fig 1.
Association and dissociation of SbtR to its consensus DNA-binding sequence.
(A) Various concentrations of SbtR were subject to BLI analysis. BLI was performed at 30°C in BLI-100 buffer (20 mM Tris-Cl [pH 7.5], 100 mM NaCl, 1 mM EDTA, and 0.05% Tween-20). Prior to the association step, streptavidin-coated biosensors were conjugated to biotinylated DNA probes containing a previously identified consensus DNA-binding motif for SbtR. In the graph shown, association occurred during the first 500 seconds, then samples were transferred to a buffer-containing well to measure dissociation. Solid lines depict lines of best fit from GraphPad Prism software. Dots represent individual BLI data points, that were taken every 0.2 seconds. (B) Kinetic data derived from the experiment shown in (A) are presented.
Fig 2.
A screenshot of results from the GraphPad Prism “Association then Dissociation” model for SbtR binding to its consensus sequence is presented.