Fig 1.
Distributions of pups born and weaned per pair with tunnel handling (n = 29 pairs) vs tail-lift with forceps (n = 30 pairs).
Frequency distribution and boxplots. (A) Total number of pups born per pair. Median difference (solid black line) was 2 pups more per pair with tunnel handling. (B) Total pups weaned per pair. Median difference (solid black line) was one more pup weaned per pair with tunnel handling.
Table 1.
Summary statistics for C57BL/6J mouse breeding pair productivity by handling method (non-aversive handling with tunnels vs tail-lift with forceps).
Fig 2.
Frequency of complete litter loss and pair non-productivity.
Complete litter loss events, as defined as disappearance of the entire litter before weaning due to pup death or disappearance, and pair non-productivity defined as no litter produced for >60 days past the previous litter weaned. Orange group = tunnel-handled pairs (n = 29); blue group = tail-lift with forceps handled pairs (n = 30).