Fig 1.
Outline of the pig pancreas anatomy.
Sites for pancreas parenchyma partition and vessel clamping marked. The tail was used for measurements. CHA, common hepatic artery; CT, coeliac trunk; GDA, gastroduodenal artery; LGA, left gastric artery; LGV, left gastric vein; PV, portal vein; SA, splenic artery; SMA, superior mesenteric artery; SMV, superior mesenteric vein; SV, splenic vein.
Fig 2.
Starting after surgery and placement of monitoring catheters, demonstrates the timeline for blood flow occlusions to the pancreas with following periods of reperfusion.
Table 1.
Number of pigs with obtained measurements for the different sensor types.
Table 2.
Systemic vital parameters and laboratory values before and after 45 min of vessel occlusion.
Fig 3.
Microdialysis parameters during arterial and venous occlusions in the porcine pancreas.
In addition, lactate and glucose were measured from systemic venous blood gases. (A) Increase in parenchymal and surface lactate after arterial and venous occlusions. Systemic lactate remained unaffected. (B) Decrease in glucose and pyruvate and increase in lactate-to-pyruvate (L/P)-ratios and glycerol, in the parenchyma and on the surface after arterial and venous occlusions. Circumferential sampling catheter refers to MDialysis 65 and unidirectional sampling catheter to OnZurf. Values presented as median and interquartile range. Maximal changes after occlusions were compared to values right before occlusions with Wilcoxon signed rank test. Significance level p < 0.05. Number of pigs included presented in Table 1. L/P-ratio, lactate-to-pyruvate ratio.
Table 3.
Pearson correlation coefficients (R) between surface and parenchymal measurements.
Fig 4.
PCO2 measurements during arterial and venous occlusions in the porcine pancreas.
Increase in parenchymal and surface pCO2 after arterial and venous occlusions. Systemic pCO2 remained unaffected. Values are presented as median and interquartile range. Maximal increases after occlusions were compared to values right before occlusions with Wilcoxon signed rank test. Significance level p<0.05.