Table 1.
Overall experimental design with specific timepoints.
Fig 1.
Antibody tittering against brain homogenate (A) and rrMOG1-125 (B) results, displayed as an absorbance for specific serum dilution factor. Results are displayed as a mean with standard deviation. P value of less than 0.0001 was presented as a “****”.
Fig 2.
Animal body weight presented as a mean with standard deviation for specified treatment group (A) with behavioral assessment results (B-D). Changes in forelimb usage was presented as a fraction of total recorded touches for groups treated with full treatment (B), VEGF only (C) and with PBS only (D). P value of less than 0.05 was presented as “*”, <0.01 as a “**”, <0.001 as a “***” and <0.0001 as a “****”.
Fig 3.
Hematoxylin/eosin stained brain tissue samples from all of the experimental groups.
Higher magnification images are taken from the lesioned area (rectangle), scale bar represents 50 microns.
Fig 4.
Eriochrome stained brain tissue samples from all of the experimental groups.
Higher magnification images are taken from the lesioned area (rectangle), scale bar represents 50 microns. The graph illustrates the lesioned area from different experimental groups. P value of less than 0.05 was presented as “*”.
Fig 5.
Immunofluorescent images taken from the lesioned area for various antigens (green) in injected groups, counterstained with DAPI (blue; A). Graphs display the endogenous IgG-positive fluorescent signal intensity in internal capsule with adjacent tissue, compared to control animals (B). Additionally, DAPI-specific signal was counted as a total area within the lesion (C) and as a mean percentage of total area within ROIs (D). P value of less than 0.05 was presented as “*”, <0.01 as a “**” and <0.001 as a “***”.