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Table 1.

The multiplex SFTSV/OT/IC RT-LAMP primer sets.

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Fig 1.

Optimization of the multiplex SFTSV/OT/IC LAMP primer set.

(A) Different concentration ratios of SFTSV, OT, and IC primer sets (1.5:2:0.6, 1.5:1.5:0.6, and 2:1.5:0.6, respectively) for SFTSV, OT, and IC plasmid mixtures (1:1:1). (B) Temperature gradient tests (59–65°C) of the multiplex SFTSV/OT/IC LAMP assay.

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Fig 1 Expand

Table 2.

Limit of detection test for the monoplex and multiplex SFTSV/OT/IC LAMP primer set.

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Table 2 Expand

Table 3.

Limit of detection tests of the multiplex SFTSV/OT/IC RT-LAMP assay, PowerChek SFTSV Real-time PCR kit and LiliF TSUTSU nested PCR kit for two-fold diluted clinical samples from patients infected with SFTSV or O. tsutsugamushi.

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Table 3 Expand

Table 4.

Comparison of clinical performance between the multiplex SFTSV/OT/IC RT-LAMP assay, PowerChek SFTSV Real-time PCR kit, and LiliF TSUTSU nested PCR kit for clinical samples from patients infected with SFTSV or O. tsutsugamushi.

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Table 4 Expand

Table 5.

Cross-reactivity of the multiplex SFTSV/OT/IC RT-LAMP assay against other fever infectious viruses.

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Table 5 Expand