Table 1.
The multiplex SFTSV/OT/IC RT-LAMP primer sets.
Fig 1.
Optimization of the multiplex SFTSV/OT/IC LAMP primer set.
(A) Different concentration ratios of SFTSV, OT, and IC primer sets (1.5:2:0.6, 1.5:1.5:0.6, and 2:1.5:0.6, respectively) for SFTSV, OT, and IC plasmid mixtures (1:1:1). (B) Temperature gradient tests (59–65°C) of the multiplex SFTSV/OT/IC LAMP assay.
Table 2.
Limit of detection test for the monoplex and multiplex SFTSV/OT/IC LAMP primer set.
Table 3.
Limit of detection tests of the multiplex SFTSV/OT/IC RT-LAMP assay, PowerChek™ SFTSV Real-time PCR kit and LiliF™ TSUTSU nested PCR kit for two-fold diluted clinical samples from patients infected with SFTSV or O. tsutsugamushi.
Table 4.
Comparison of clinical performance between the multiplex SFTSV/OT/IC RT-LAMP assay, PowerChek™ SFTSV Real-time PCR kit, and LiliF™ TSUTSU nested PCR kit for clinical samples from patients infected with SFTSV or O. tsutsugamushi.
Table 5.
Cross-reactivity of the multiplex SFTSV/OT/IC RT-LAMP assay against other fever infectious viruses.