Fig 1.
A diagram of the two-phase workflow to determine qPCR results.
Fig 2.
Heat map illustrating the concentration of eDNA detected in water samples from November 2016 to August 2019.
Each depth of color indicates a percentile of concentration level (interval = 0.2). A darker color represents a higher concentration level for positive detection (in green) and uncertain detection (in purple). Grey represents samples with no amplification, and categorized as negative detection.
Table 1.
A summary of amplified samples across the three stream categories: Known Population (KP), Historical Record (HR), Unknown (UN).
Table 2.
Summary of trapping surveys throughout the eDNA sampling period at streams with known populations (KP).
There were positive sightings of Platysternon megacephalum using active searching at all streams.