Fig 1.
Phylogenetic analysis based on a multilocus dataset, showing the position of the newly described genus (Thainema gen. nov.).
The tree is based on Bayesian topology, and the support values are given for both the Bayesian posterior probabilities plus the bootstrap values for the maximum likelihood tree. The scale bar represents the number of nucleotide substitutions per site. Reference sequences of the taxa are marked with an asterisk (*). The legend indicates the families and previously proposed family in the tree.
Table 1.
16S rRNA dissimilarity among Thainema strains (displayed values are in %).
Table 2.
16S rRNA genetic similarity among the Thainema gen. nov. and other phylogenetically related groups.
Fig 2.
Helices 23 showing the molecular determinations of the proposed families.
Fig 3.
Helices 27 showing the molecular determinations of the proposed families.
Table 3.
Nucleotide variation between families within the order Synechococcales.
Fig 4.
Phylogenetic tree reconstructed using Beast, based on the 16S rRNA gene sequence.
The support values illustrate Bayesian posterior probabilities. Each column on the right shows a different species delimitation method, and each rectangle indicates a separate species. The legend indicates the proposed families belonging to the order Synechococcales.
Fig 5.
Secondary structure of the D1-D1´ and Box-B helices in the ITS region of strains CCALA 10287 and UTEX SP44.
(A) D1-D1´ helices. (B) Box-B helices.
Fig 6.
Light microscope view of Thainema salinarum.
The golden granules at cross-walls and sheaths are observed. Scale bar = 10 μm.
Fig 7.
Micrographs of Thainema salinarum obtained with a transmission electron microscope (TEM).
(A, B) Cells with parietal thylakoids, granules, and sheath. Scale bar = 0.5 μm. (C) Longitudinal section of filaments showing the presence of parietal thylakoids. Scale bar = 1 μm. (D) Short filament showing a round apical cell. Scale bar = 5 μm.