Fig 1.
Steps of the extended QbD method.
Table 1.
Factor values for samples according to 23 full factorial design.
Table 2.
Encapsulation efficiency results.
Fig 2.
The response surfaces (alginate concentration on zero level) and the predicted values of (a) enzyme activity; (b) EE%; (c) particle size; (d) α-helix content.
Table 3.
Particle size and zeta potential results.
Fig 3.
Representative TEM pictures of alginate layered NPs (Sample 2).
Table 4.
The determined enzyme activity (%) values of the different samples.
Fig 4.
FT-IR spectra of the different samples.
Fig 5.
Deconvolution of FT-IR spectrum of LYS, precipitated LYS and the samples.
Table 5.
The α-helix content of the samples.
Fig 6.
Results of CD spectroscopy for LYS, precipitated LYS and core-shell NPs.