Fig 1.
Development of the RT-LAMP method for HPeVs.
(a) The HPeV3 genome and scheme of the combinations of the primers for HPeVs. Three primer sets for RT-LAMP were designed on the 5′ UTR of HPeV3. (b) Results of RT-LAMP using the three primer sets. Tubes containing HPeV3 RNAs show a bright fluorescence, compared with tubes with distilled water. DW, distilled water; HPeV, human parechovirus; HPeV3, human parechovirus A3; RT-LAMP, reverse transcription-loop-mediated isothermal amplification; UTR, untranslated region.
Table 1.
Primer information for RT-LAMP of HpeVs.
Fig 2.
Results of RT-LAMP using different viral genomes.
Tubes containing HPeV1 and HPeV3 show positive results, whereas the other tubes show negative results. HPeV1, human parechovirus type 1; HPeV3, human parechovirus type 3; Neg, negative; Pos, positive; RT-LAMP, reverse transcription loop-mediated isothermal amplification.
Fig 3.
Representative picture of the results of RT-LAMP by using clinical samples.
The stool samples of two patients (Patient #1 and Patient #2) show negative results. The stool samples of four patients (Patients #3–#6) show positive results for RT-LAMP. This figure is from primer Set A. The results of nested PCR for HPeV3 corresponded completely to the results of RT-LAMP. HPeV3, human parechovirus A3; Neg, negative; PCR, polymerase chain reaction; Pos, positive; RT-LAMP, reverse transcription loop-mediated isothermal amplification.