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Fig 1.

Gram stain of the selected Bacillus strain showing gram-positive rods.

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Fig 2.

16srRNA sequence of Bacillus marisflavi.

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Fig 3.

Molecular phylogenetic analysis by maximum likelihood method.

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Fig 4.

Synthesis of AgNPs by B.marisflavi.

A: Zobell marine broth (control) + 1mM Silver nitrate (no color change); B: Culture supernatant of B. marisflavi + 1mM Silver nitrate (visible color change from yellow to brown).

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Fig 5.

UV-VIS spectrum showing maximum absorption between 420-430nm.

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Fig 6.

AFM results showing spherical nanoparticles.

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Fig 7.

SEM image of silver nanoparticles of nano size averaging 78.77 nm.

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Fig 8.

DLS results showing the diameter and polydispersity index of the silver nanoparticles.

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Fig 9.

FTIR results showing the presence of different chemical groups in the silver nanoparticles.

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Table 1.

Functional groups corresponding to different absorption peaks obtained for the AgNPs.

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Fig 10.

Mortality curves for ovicidal activity of AgNPs synthesized by Bacillus marisflavi against the eggs of Ae. aegypti, Cx. quinquefasciatus and An. Stephensi.

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Fig 11.

Mortality curves for pupicidal activity of AgNPs synthesized by Bacillus marisflavi against the pupae of Ae. aegypti, Cx. quinquefasciatus and An. Stephensi.

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Fig 12.

Mortality curves for larvicidal activity of AgNPs synthesized by Bacillus marisflavi against 3rd instar larvae of Ae. aegypti, Cx. quinquefasciatus and An. Stephensi.

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Fig 13.

Mortality curves for larvicidal activity of AgNPs synthesized by Bacillus marisflavi against 4th instar larvae of Ae. aegypti, Cx. quinquefasciatus and An. Stephensi.

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Table 2.

Ovicidal activity of AgNPs synthesized by Bacillus marisflavi against the eggs of Ae. aegypti, Cx. quinquefasciatus and An. stephensi.

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Table 3.

Lethal concentrations, R2, Regression equations and χ2 values for Ovicidal activity of AgNPs synthesized by Bacillus marisflavi against Ae. aegypti, Cx. quinquefasciatus and An. stephensi.

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Table 4.

Pupicidal Activity of AgNPs synthesized by Bacillus marisflavi against the pupae of Ae. aegypti, Cx. quinquefasciatus and An. stephensi.

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Table 5.

Lethal concentrations, R2, Regression equations and χ2 values for pupicidal activity of AgNPs synthesized by Bacillus marisflavi against the pupae of Ae. aegypti, Cx. quinquefasciatus and An. stephensi.

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Table 6.

Larvicidal Activity of AgNPs synthesized by Bacillus marisflavi against Ae. aegypti, Cx. quinquefasciatus and An. stephensi.

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Table 7.

Lethal concentrations, R2, Regression equations and χ2 values for larvicidal activity of AgNPs synthesized by Bacillus marisflavi against Ae. aegypti, Cx. quinquefasciatus and An. stephensi.

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Table 7 Expand