Fig 1.
Metaphase (A) and karyotype (B) of S. tyrannus with 2n = 68, obtained with Giemsa conventional staining.
The red arrows in (A) indicate the sex chromosomes. Scale bar: 5μm.
Table 1.
Karyotype of S. tyrannus described by Tagliarini et al. [1] and at this study.
Fig 2.
Representative results of FISH experiments using G. gallus chromosome-specific probes corresponding to pairs GGA1 to GGA5 in S. tyrannus karyotype.
Red and green signals represent probes labelled with Cy3 or FITC, respectively. Scale bar: 5μm.
Table 2.
Results of hybridizations with G. gallus probes showing the homology between GGA probes in the karyotype of S. tyrannus (STY).
Fig 3.
Representative results of hybridizations with some G. gallus BACs probes in the karyotype of S. tyrannus. (A1 and A1.1) chicken BAC17 was the only one to hybridize to different chromosomes.
Red signals represent probes labeled with Cy3, corresponding to the proximal region (px); Green signals represent probes labeled with FITC, corresponding to the distal region (d). Arrows indicate the signals. Scale bar: 5μm.
Table 3.
Summary of the results of experiments using GGA BACs on the karyotype of S. tyrannus (* = BACs marking the same segment in STY karyotype; px = proximal region; d = distal region; p = short arm; q = long arm).
Fig 4.
Idiogram representing the homology between the S. tyrannus chromosomes and the macrochromosome chromosome-specific probes and microchromosomes BAC clones from G. gallus.
Empty boxes mean no signal detected in those chromosomes with the set of probes used. BACs corresponding to pairs 20 and 22 (*) was not used or did not produce any detectable signals, respectively.