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Fig 1.

Methodology steps for building the machine vision algorithm for pain assessment in horses.

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Fig 2.

Horse face regions and pain levels for evaluation by computer modeling.

Ears: Considers ear position, distance between them and direction. Eyes: Considers size of the orbital area, muscular tension above the eye, visibility of underlying bone surfaces. Mouth and nostrils: Considers level of mouth and nostrils straining, tension, size of mouth-collum (line between the upper and lower lips) and nostril dilation.

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Fig 3.

Window application software for the labeling process.

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Table 1.

Composition of final image database.

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Table 2.

Model architecture based on the convolutional neural network that was used to classify the pain level for each evaluated parameter (ears, eye and mouth and nostrils).

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Table 3.

Hyperparameters used in the final classifier model for each evaluated parameter (ears, eye, and mouth and nostrils).

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Table 4.

Confusion matrix between the data classified by the CNN-based model and HGS for the ears images.

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Table 5.

Confusion matrix between the data classified by the CNN-based model and HGS for the eyes images.

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Table 6.

Confusion matrix between the data classified by the CNN-based model and HGS for the mouth and nostrils images.

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Table 7.

Confusion matrix between the data classified by the ANN-based classifier and HGS, described on three levels of pain.

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Table 8.

Confusion matrix between the data classified by the ANN-based classifier and HGS, described on two levels of pain.

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