Fig 1.
Electrophoretogram comparison of plasma protein fractions.
A visual representation of the plasma protein fraction distribution amongst three representatives from each age classes. The top panel shows the protein as they appeared on the gel following electrophoresis, and the bottom panel shows a graphic representation of the concentration differences. Juvenile (A), sub-adult (B), and adult (C).
Fig 2.
Model predictions for plasma protein electrophoresis values based on year and location.
Model predictions with 95% confidence intervals for plasma protein electrophoresis values in free-living Blanding’s turtles (Emydoidea blandingii) based on year and location. Model estimates were produced by top-ranking general linear mixed models (see Table 2). SB = Spring Bluff-Chiwaukee Prairie, IB-N = Illinois Beach North, IB-S = Illinois Beach South.
Fig 3.
Model predictions for plasma protein electrophoresis values based on year, location, month, and BCI.
Model predictions with 95% confidence intervals for plasma protein electrophoresis values in free-living Blanding’s turtles (Emydoidea blandingii) based on year, location, month, and body condition index (BCI). Model estimates were produced by top-ranking general linear mixed models (see Table 2).
Fig 4.
2018 and 2019 spatial clusters of plasma protein electrophoresis values in free-living Blanding’s turtles (Emydoidea blandingii).
Study site map displaying hot and cold spots for each analyte in 2018 and 2019. Hot spots are areas with higher plasma protein concentrations, while cold spots are areas with lower plasma protein concentrations. SBCP, the managed site, is the northernmost territory on the map followed by the North unit of IBSP as the central territory and the South unit of IBSP as the southernmost territory. Evaluated using Gettis-Ord Gi* models. Map obtained from USGS National Map Viewer. TP = total protein (g/L), PreALB = prealbumin (g/L), ALB = albumin (g/L), Alpha1 = alpha 1 globulins (g/L), Alpha2 = alpha 2 globulins (g/L), Beta = beta globulins (g/L), Gamma = gamma globulins (g/L), A:G = albumin / globulin.
Table 1.
Protein electrophoresis values that vary by month and the presence of plastron abnormalities in free-living Blanding’s turtles (Emydoidea blandingii).
Model estimates were produced by top-ranking general linear mixed models (see Table 2).
Table 2.
Top models for predicting protein electrophoresis values in free-living Blanding’s turtles (Emydoidea blandingii) based on Akaike’s information criterion, corrected for sample size (AICc).
Table 3.
Protein electrophoresis values that vary by age class in free-living Blanding’s turtles (Emydoidea blandingii).
Model estimates were produced by top-ranking general linear mixed models (see Table 2).
Table 4.
Protein electrophoresis values that vary by sex and gravidity in free-living Blanding’s turtles (Emydoidea blandingii).
Model estimates were produced by top-ranking general linear mixed models (see Table 2).
Table 5.
Protein electrophoresis values that vary based on the presence of physical examination abnormalities in free-living Blanding’s turtles (Emydoidea blandingii).
Model estimates were produced by top-ranking general linear mixed models (see Table 2). Top models for relative and absolute alpha 1 globulins contained “carapace”, however no contrasts for this predictor were significant therefore not reported.
Table 6.
Summary data including data distribution, measure of central tendency (mean for normally distributed variables, median for non-normally distributed variables), measure of dispersion (standard deviation for normally distributed variables, 10th– 90th percentiles for non-normally distributed variables), and reference intervals for plasma protein electrophoresis in free-living, apparently healthy Blanding’s turtles (Emydoidea blandingii).