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Fig 1.

PRISMA diagram of the study selection process.

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Fig 2.

Subgroups of studies according to the methods used for HER2 positivity determination.

Abbreviations: IHC = Immunohistochemistry, ISH = In Situ Hybridization.

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Table 1.

Main characteristics of the included studies.

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Fig 3.

Prevalence of HER2 overexpression according to ASCO/CAP-compliant guidelines.

Abbreviations: ASCO = American Society of Clinical Oncology, CAP = College of American Pathologists, DL = Der Simonian and Laird, ME = mixed effects.

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Fig 4.

Subgroup analysis by histologic subtype.

All studies. Abbreviations: DL = Der Simonian and Laird, ME = mixed effects.

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Fig 5.

Studies that included both squamous and non-squamous histology.

Abbreviations: DL = Der Simonian and Laird, ME = mixed effects.

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Fig 6.

Subgroup analysis by World Health Organization geographic region.

Abbreviations: DL = Der Simonian and Laird, ME = mixed effects.

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Fig 7.

Subgroup analysis by primary antibody brand.

Abbreviations: DL = Der Simonian and Laird, ME = mixed effects.

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Fig 8.

Relationship between the year of study publication, size, and HER2 overexpression prevalence.

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Fig 9.

Prevalence of HER2 amplification according to compliance with ASCO/CAP guidelines.

Abbreviations: ASCO = American Society of Clinical Oncology, CAP = College of American Pathologists, DL = Der Simonian and Laird, ME = mixed effects.

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Fig 10.

HER2 overexpression prevalence in selected histologic subtypes.

Abbreviations: DL = Der Simonian and Laird, ME = mixed effects.

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Table 2.

Summary of the main findings of the review.

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Table 2 Expand