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Fig 1.

Plant material was sampled from the structure (coarse outer material) and lining (fine inner material) of this Song Sparrow (Melospiza melodia maxillaris) nest specimen (MVZ1:Egg:1613).

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Fig 2.

Samples were systematically selected from the structure (blue oval) and lining (red oval) at equally spaced locations (diamonds).

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Fig 2 Expand

Table 1.

Bird nest specimens selected for sampling.

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Table 2.

Taxonomic identifications of samples.

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Table 2 Expand

Fig 3.

Difference in sequence quality (HQ%) between samples identified as plant and green algae.

Significant correlations are indicated as follows: ** p < 0.01.

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Fig 3 Expand

Table 3.

Characteristics of successfully sequenced samples.

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Table 3 Expand

Fig 4.

Differences in sample characteristics (a. dry mass, b. DNA concentration, and c. DNA purity) between samples with sequencing success or failure. Significant correlations are indicated as follows: * p < 0.05, ** p < 0.01, NS. = not statistically significant.

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Fig 4 Expand