Fig 1.
Kaplan-Meier survival curve and Pearson correlation coefficient based on the categorization of MIF and DDT expressions in the UCSC Xena platform.
Fig 2.
(A) In vitro cytotoxicity test of 4-IPP in a time- and dose-dependent manner. (B) Survival curves of 528NS cells with various treatments are indicated. The effects of treatment were evaluated after treatment with the single and combinational application of 50 μM 4-IPP and X-ray irradiation (6Gy). (C) Protein expression by Western blot in 528NS cells when treated by single or combinational administration of 4-IPP and radiation.
Fig 3.
Immunohistochemical analysis of subcutaneous xenograft mouse model.
Scale bar, 100 μm.
Fig 4.
Cleaved caspase-3 and Ki-67 immunohistochemical stain on ex vivo tissue explants.
(A) Representative images for cleaved caspase-3 and Ki-67 are shown. Scale bar, 100 μm. (B) The summary of the quantitative analysis results for cleaved caspase-3 and (C) Ki-67 is shown. The data represents the mean value of the number of cleaved caspase-3-positive cells per arbitrary unit (n = 5) and the percentage of Ki-67-positive stained area for each selected tissue area (n = 5). P values: **** p < 0.0001.
Fig 5.
The effect of 4-IPP and radiation in the subcutaneous xenograft model.
(A) Tumor size curves of different groups of mice during treatment. (B) Tumor weights and (C) photo of extracted tumors after treatment. P values: *p < 0.05, **p < 0.01, ***p < 0.001, **** p < 0.0001.