Table 1.
Summary of the correlation coefficient, accuracy, precision, LOD and LOQ values for the proposed method.
Fig 1.
Calibration curve showing the linearity of the method: Neu5Ac.
Fig 2.
A chromatogram of cultured HeLa cells via using the optimised HPLC method.
Absence of significant interference peaks at the retention times (6.344 minutes with a flow rate of 0.4 mL/min.) of Neu5Ac has been highlighted in red.
Table 2.
Results for accuracy (n = 3) for the proposed method to determine Neu5Ac level.
Table 3.
Results of precision for the proposed method to determine Neu5Ac level.
Fig 3.
The changes in the total sialic acid level according to various concentration of deoxycholic acid (0 μM, 75 μM and 150 μM) added for HeLa (Graph A) and HuCCT1 (Graph B) cells.
The values are mean ± SD, n = 6. The asterisks indicate the total sialic acid level for 75 μM and 150 μM were statistically significantly different to the control Neu5Ac level: *P < 0.05.