Fig 1.
Flow-chart of study design.
Table 1.
Participant characteristics.
Fig 2.
OPLS-DA model of AM operators year 1 after selecting proteins with VIP values ≥ 1.2 and VIP values > standard error (SE).
A: Score plot of participating AM operators at the beginning and end of the week. The x-axis depicts the predictive component most important for separating samples collected Monday (grey circles) vs Friday (black circles) while the y-axis represent the orthogonal component showing in-group variation. R2 = 0.97, Q2 = 0.914, CV-ANOVA p-value = 0.007. B: Loading plot showing the underlying protein separation (X-variables, grey circles) in relation to sampling time point (Y-variables, black circles). FGB—Fibrinogen beta chain, GOLM1—Golgi membrane protein 1, IGHV1-69—Immunoglobulin heavy variable 1–69, JCHAIN—Immunoglobulin J chain, IGKC–Immunoglobulin kappa constant, IGKV3D-11 –Immunoglobulin kappa variable 3–11, LPO–Lactoperoxidase, PIP—Prolactin-inducible protein, S100A6—Protein S100-A6, SCB1A1 –Uteroglobin, WFDC2—WAP four-disulfide core domain protein 2, VTN–Vitronectin.
Table 2.
Proteins obtained from orthogonal partial least squares-discriminant analysis when comparing additive manufacturing operators Monday vs Friday year1.
Fig 3.
Comparison of protein differences in nasal lavage fluid collected from additive manufacturing operators year 1 and year 2.
Values are median (range), LFQ–label free quantification, * p<0.05 Wilcoxon matched pairs test.
Fig 4.
OPLS-DA model of welders year 1 after selecting proteins with VIP values ≥ 1.2 and VIP values > standard error (SE).
A: Score plot of participating welders at the beginning and end of the week. The x-axis depicts the predictive component most important for separating samples collected Monday (grey circles) vs Friday (black circles) while the y-axis represent the orthogonal component showing in-group variation. R2 = 0.84, Q2 = 0.20, p > 0.05. B: Loading plot showing the underlying protein separation (X-variables, grey circles) in relation to sampling time point (Y-variables, black circles). ANXA1—Annexin A1, CALM3—Calmodulin-3, GSTP1—Glutathione S-transferase P, IGHV3-7—Immunoglobulin heavy variable 3–7, IGJ—Immunoglobulin J chain, IGLC3—Immunoglobulin lambda constant 3, MUC19—Mucin-19, PIGR—Polymeric immunoglobulin receptor, PTMA—Prothymosin alpha, S100A6—Protein S100-A6, TXN–Thioredoxin.
Table 3.
Proteins obtained from orthogonal partial least squares-discriminant analysis when comparing welders Monday vs Friday year1.