Fig 1.
Transmission electron micrograph of Pontibacillus sp. ALD_SL1 (A) and Psychroflexus sp. ALD_RP9 (B). Cells from an overnight culture were stained with phosphotungstic acid (A) or uranyl acetate (B). A: rod-shaped Pontibacillus sp. ALD_SL1 with two long peritrichious flagella. B: Psychroflexus sp. ALD_RP9 cells are rod-shaped and lack flagella or pili. EPS are visible as a web of thin filaments broadly surrounding the cell. The scale bars in the bottom left measure 0.5 μm (A) and 0.1 μm (B).
Table 1.
Morphological, growth and enzymatic characteristics of Pontibacillus sp. ALD_SL1 and Psychroflexus sp. ALD_RP9.
Fig 2.
Neighbor-joining phylogenetic tree of the Pontibacillus and Psychroflexus genera.
The tree includes 16S rRNA gene sequences from genomes (*), and genome scaffolds (S and scaffold number). The optimal tree with the sum of branch length 1.05278265 is shown. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1,000 replicates) are shown next to the branches. Bootstrapping values at the branches indicate the mean result of the neighbor-joining, maximum-likelihood and maximum-parsimony method. Evolutionary distances were calculated using the Kimura 2-parameter model with Archaeoglobus fulgidus L3 as an outgroup.
Fig 3.
Genome features and comparison of Pontibacillus sp. ALD_SL1 and Psychroflexus sp. ALD_RP9 with all available scaffolds and genomes of their genus.
The image was generated using BRIG [32] and a nucleotide blast for genome comparison. Ring 1: selected genome features from the functional annotation with BlastKOALA [33]. Ring 2: genomic islands (red) predicted with IslandViewer 4 [37] and prophages (orange) identified using PHASTER [38]. Rings 3–9 (A) and 3–11 (B): available genomes and scaffolds for each genus.
Table 2.
Genome compositions.
Fig 4.
Relatedness of Pontibacillus (A) and Psychroflexus (B) genomes. All available genome assemblies of each genus were aligned and compared using ANIm in pyANI v0.2.10. [31]. The level of genome completeness is indicated in upper case: contig (C), scaffold (S), full genome (G), genus type species (T).
Fig 5.
Key aspects of the Pontibacillus sp. ALD_SL1 (A) and Psychroflexus sp. ALD_RP9 (B) metabolism. Metabolic pathways and capabilities were reconstructed from the complete genomes against the KEGG database using BlastKoala [33] and the KEGG Mapper [34].
Table 3.
Functional assignment of genes into KEGG categories.