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Table 1.

Five specimens of Thamnophis sirtalis included in this study.

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Fig 1.

DNA preservation.

DNA concentration measurement and DNA preservation metrics listed according to specimen age. Endo %, endogenous DNA content; Length, average length (in base pairs) of mapped reads; C-T %, C-to-T deamination damage fraction recorded at first position in the 5’end. Bars represent average values of two replicate DNA extracts with the range shown.

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Table 2.

DNA preservation.

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Table 2 Expand

Table 3.

Linear mixed effects model.

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Fig 2.

Human DNA contamination.

Fraction of sequences that could be mapped to the human reference genome listed according to specimen age. Bars represent average values of two replicate DNA extracts and the range is shown.

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Fig 2 Expand

Fig 3.

Metagenomic profiling.

Bacterial metagenomic diversity visualized in Krona and Venn (lower right) plots for the three tissue types. The diversity is presented at species-level and includes only NCBI refseq taxa with >5 sequence matches. Colors denote taxonomic groups found within the various bacterial phyla as according to Krona visualization. Blanks denote negative extraction control.

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