Table 1.
Number of cows with a positive and negative test result.
Table 2.
Variance components, standard errors (SE), and h2 estimates for the diagnostic tests and their combinations.
Fig 1.
Number of SNPs associated with PTB infection status.
The total number of SNPs with moderate (A), or high (B) association with PTB infection status determined by ELISA (E), tissue PCR (P), and tissue culture (C) combinations. Animals were considered cases when they were positive to all the tests, and controls when they were negative to any (+/), or all of them (+/-). A moderate threshold for the association between a SNP and a phenotype was set at a significance level (α) between P = 5 × 10−5 and P = 5 × 10−7. The high threshold for evidence of an association was set at P < 5 × 10−7.
Fig 2.
Manhattan plots showing–log10(P-values) of association between every single SNP and phenotype.
Each dot represents the result from the test association for a single SNP. Animals were considered cases when they were positive to all the tests and controls when they were negative to any (+/), or all of them (+/-). Chromosomes localization of the SNPs associated with ELISA-tissue culture-tissue PCR (+/-) (A), ELISA-tissue culture-tissue PCR (+/) (B), ELISA-tissue culture (+/) (C), and ELISA-tissue PCR (+/) (D) is indicated on the x-axis. The horizontal red line is drawn at–log10 (2.5 × 10−8), and the horizontal blue line at–log10 (5 × 10−7) to show the high level of significance.
Table 3.
Quantitative trait loci (QTLs) surpassing the significance threshold (P< 5 × 10−7) for evidence of an association with MAP infection status.
Table 4.
Odds ratio (OR) for some the SNPs associated with a positive ELISA-tissue PCR-tissue culture diagnosis (+/-)(P < 5 x 10−7).