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Table 1.

The new primer set flanking the (TTTAT)3-5 microsatellite located within the intron 6 of the LDHA gene.

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Fig 1.

PCR analyses of five identified genotypes (A-E) using agarose gel electrophoresis (A) and urea-PAGE (B). Lines: M. O’RangeRuler 20 bp DNA Ladder (Thermo Scientific). A. (TTTAT)3/(TTTAT)3 homozygote. B. (TTTAT)4/(TTTAT)4 homozygote. C. (TTTAT)3/(TTTAT)4 heterozygote. D. (TTTAT)4/(TTTAT)5 heterozygote. E. (TTTAT)5/(TTTAT)5 homozygote. PCR plausible heteroduplexes marked by stars.

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Fig 1 Expand

Fig 2.

The sequencing results for the (TTTAT)3-5 microsatellite polymorphism in the LDHA gene.

A. (TTTAT)3/(TTTAT)3 homozygote. B. (TTTAT)4/(TTTAT)4 homozygote. C. (TTTAT)3/(TTTAT)4 heterozygote. D. (TTTAT)4/(TTTAT)5 heterozygote. E. (TTTAT)5/(TTTAT)5 homozygote.

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Fig 2 Expand

Fig 3.

Results of qPCR-HRM analysis.

A. The normalized HRM profile of five identified genotypes. B. The difference graph (sample A genotype 3/3 was selected as reference). A. (TTTAT)3/(TTTAT)3 homozygote. B. (TTTAT)4/(TTTAT)4 homozygote. C. (TTTAT)3/(TTTAT)4 heterozygote. D. (TTTAT)4/(TTTAT)5 heterozygote. E. (TTTAT)5/(TTTAT)5 homozygote.

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Fig 3 Expand