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Fig 1.

Timeline of interventions and points at which drugs were given and pain ratings were performed.

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Table 1.

Methods for statistical validation of the Unesp-Botucatu horse acute pain scale (UHAPS), Composite Pain Scale (CPS), and unidimensional scales to assess postoperative pain in horses.

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Table 1 Expand

Table 2.

Repeatability of the Unesp-Botucatu horse acute pain scale (UHAPS), Composite Pain Scale (CPS), and unidimensional scales to assess perioperative pain in horses.

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Table 3.

Observer matrix correlation for the Unesp-Botucatu horse acute pain scale (UHAPS) and the Composite Pain Scale (CPS) to assess perioperative pain in horses.

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Table 4.

Reproducibility of the Unesp-Botucatu horse acute pain scale (UHAPS), Composite Pain Scale (CPS), and unidimensional scales to assess perioperative pain in horses.

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Table 5.

Load, eigenvalue, and variance of the Unesp-Botucatu horse acute pain scale (UHAPS) and the Composite Pain Scale (CPS) submitted to principal component analysis.

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Table 6.

Criterion validity based on the correlation between the Unesp-Botucatu horse acute pain scale (UHAPS) and the Composite Pain Scale (CPS) vs unidimensional scales to assess perioperative pain.

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Table 7.

Item-total correlation and internal consistency, specificity, and sensitivity for the Unesp-Botucatu horse acute pain scale (UHAPS).

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Table 8.

Item-total correlation and internal consistency, specificity, and sensitivity for composite pain scale.

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Fig 2.

Box plots of the total scores of the Unesp-Botucatu horse acute pain scale (UHAPS) over time.

M0 –before surgery; M1 –up to 4 hours after surgery; M2—up to 3 hours after analgesic treatment; M3–24 hours after surgery. Small letters represent statistically significant differences (a > b).

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Fig 3.

Box plots of the total scores of the Composite Pain Scale (CPS) over time.

M0 –before surgery; M1 –up to 4 hours after surgery; M2—up to 3 hours after analgesic treatment; M3–24 hours after surgery. Small letters represent statistically significant differences (a > b).

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Fig 3 Expand

Table 9.

Median (range) scores of the Unesp-Botucatu horse acute pain scale (UHAPS), the Composite Pain Scale (CPS), and unidimensional scales in the perioperative period in horses.

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Fig 4.

Distribution of scores for each item of the Unesp-Botucatu horse acute pain scale (UHAPS) at all perioperative moments.

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Fig 5.

Distribution of scores for each item of the Composite Pain Scale (CPS) at all perioperative moments.

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Fig 6.

ROC curve and area under the curve (AUC) for the Unesp-Botucatu horse acute pain scale (UHAPS) (left) and the Composite Pain Scale (CPS) (right).

*ROC: Receiver operating characteristic curve; AUC 1 excellent; AUC 0.9–0.8 very good; AUC 0.8–0.7 good; AUC 0.7–0.6 sufficient; AUC 0.6–0.5 bad; AUC < 0.5 not-discriminative.

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Table 10.

Youden index based on highest sensitivity and specificity for the Unesp-Botucatu horse acute pain scale (UHAPS), the Composite Pain Scale (CPS), and unidimensional scales.

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Table 11.

Number of horses requiring rescue analgesia would be indicated based on clinical experience and the Youden index.

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