Table 1.
Mycobacterial isolates (lineages/sub-lineages) used for each experiment.
Fig 1.
Utilization of different carbon sources on Löwenstein-Jensen media slants.
Growth was compared as degree of positivity (WHO standard for grading Mtb growth on L-J) on both glycerol and pyruvate supplemented media slants. Two-way analysis of variance (ANOVA) considering Time as a factor showed significant difference between the 3 lineages on glycerol (p<0.001) and on pyruvate (p = 0.005) supplemented media.
Fig 2.
Time to positivity (TTP) on 7H11 media.
The average time to positivity was measured in terms of the number of days it took for the first colonies to appear for CFU determination and plotted against the various media compositions.
Fig 3.
The average logCFU/ml was plotted against the different supplemented media.
Fig 4.
Optical density at 600nm was measured at the data points (days) and indicated by the symbols. The data points are the averages of individual strains belonging to the 3 different lineages.
Table 2.
Mutational analysis of genes that may be associated with growth rate.
Fig 5.
Comparison of nitrite concentration.
The individual isolates are represented by the black points in the boxplot.
Table 3.
Genomic analysis for genes that may be involved in nitric oxide.
Fig 6.
Comparison of nitrate concentration.
The individual isolates are represented by the black points in the boxplot.
Fig 7.
Comparison of urease activity of the three different lineages.
A negative control depicted by the blue line was only urea broth. H37Rv was included in the experimental set-up as a positive control (black line).
Table 4.
Genomic analysis for urease activity test.
Fig 8.
A negative control depicted by the blue line had only the Tween hydrolysis reagent. Mycobacterium aurum represented by the purple line was included in the experimental set-up as a positive control.
Table 5.
Thiophen-2-carboxylic acid hydrazide (TCH) susceptibility profile.