Fig 1.
Map showing the different ranges and area names of the Sundarbans where the specimens were collected.
Table 1.
List of primers and thermal conditions used in PCR amplification for DNA barcoding.
Table 2.
Genetic distance of COI gene of different taxonomic levels of fish based on the K2P distance model.
Fig 2.
Sequence divergence between all sequences at species and genus level.
Distance model: Kimura 2-Parameter, COI marker, complete deletion, GPS alignment Kalign.
Fig 3.
Normalized divergence histogram plots shows species (pink) against the genus divergences (green).
Distance model: Kimura 2-Parameter, COI marker, Complete Deletion deletion, GPS alignment Kalign.
Fig 4.
Substitution saturation plot of COI gene region of fishes.
The x-axes ’F84 distance’ is based on the F84 substitution model and is expected to increase linearly with divergence time. The vertical axis is for the observed proportion of transitions (S) and transversions (V), respectively.
Fig 5.
Substitution saturation plot of 16S rRNA gene region of fishes.
The x-axes ’F84 distance’ is based on the F80 substitution model and is expected to increase linearly with divergence time. The vertical axis is for the observed proportion of transitions (S) and transversions (V), respectively.
Fig 6.
Substitution saturation plot of crustacean COI sequences.
The x-axes ’F84 distance’ is based on the F80 substitution model and is expected to increase linearly with divergence time.